In 2017 the Canadian Wildlife Federation (CWF) led a national citizen science initiative, supported by the Canadian government, celebrating 150 years of Confederation. This engaged thousands of people in a participatory exploration of biodiversity. A Bioblitz is a race against the clock that allows the public to engage with species experts to document as many living things as possible in a given area. CWF coordinated 35 bioblitzes including 10 scientific inventories and 5 flagship events across all provinces and territories, while the Parks Canada Agency hosted 22 additional ones. Data was collected using iNaturalist Canada to record verifiable nature observations using a digital camera or the iNaturalist app. The Bioblitz Canada 150 dataset as well as the millions of other observations, are publicly available for conservation and education. As a lasting legacy, CWF has created “bioblitz-in-a-box” which is an online toolkit to carrying out a bioblitz event.