A method for ecological breeding of giant salamanders includes establishing an environment mimicking their natural habitat, selecting and nurturing broodstock, artificial ovulation induction, insemination, incubation, and seedling rearing. This involves creating a model based on the natural habitat’s ecological and water factors to determine optimal construction parameters for streams and caves, ensuring ideal living conditions. Selective breeding in these environments promotes natural reproduction and dietary trials to establish the best nutritional plans, enhancing male sperm quality and female egg-bearing capacity.