In September 2022 a training was held in Vietnam’s Cúc Phương National Park on the recently developed IUCN guidance on Prevention, Detection, Response and Recovery from Disease Risks in Protected and Conserved Areas and accompanying One Health Principles for Sustainable Tourism. The training was designed for professionals, wildlife handlers and park rangers to introduce the One Health concept, IUCN guidance, sampling, human safety, risk assessment, biosafety, personal protective equipment (PPE), and biohazard waste disposal. Forty participants from Cát Tiên, Pù Mát and Cúc Phương National Parks took part in the two-day training. The field experience and expertise of the training team and adaptation to the relevant context ensured practical application of the guidance. Sessions such as the ‘Glo Germ’ test, demonstrating the importance of proper PPE donning and doffing and handwashing procedures, helped make the training interactive and reinforce key concepts.