In India, despite an ever-increasing interface between animals and humans resulting in human-wildlife conflict (HWC) and risks of zoonotic diseases, measures to address the health of animals and humans are implemented by wildlife, veterinary and public health sector institutions through separate channels, which is inhibiting operationalization of One Health approach. The much needed mechanism for such coordination has been developed  and operationalised, embedded in an overarching capacity development plan, by the Indo-German Cooperation Project on HWC Mitigation in India. The project has successfully integrated One Health into the national HWC mitigation plan and guidelines, established a system of collaborative training courses, with forest, veterinary, agriculture and public health sector institutions and experts, which has resulted in a joint training curriculum and has strengthened specialised rapid response teams at field-level.