Since 1993, a joint Canada–US not-for-profit organisation, the Yellowstone to Yukon Conservation Initiative, has brought partners together to achieve a vision of connecting and protecting the region so that people and nature can thrive. More than 400 different entities have been or currently are engaged in collaborative conservation that advances the vision across this ecological network. These include conservation groups, local landowners, Indigenous entities, businesses, government agencies, funders and donors, and scientists. Conservation progress across the Y2Y region is due to the collective work of these different groups. Conservation priorities range from protecting areas important for biodiversity and restoring and maintaining areas between protected areas for ecological connectivity, to directing development away from areas of biological importance and promoting policies and practices for people and wildlife to live in harmony across the region.