Sport can have significant negative impacts on biodiversity, through the construction and use of sports venues and the staging of sporting events. Understanding and managing the potential negative impacts and opportunities for conservation is vital for ensuring that sports venues and sporting events deliver successfully both from the financial and operational standpoint. This guide is designed to help decision makers understand these potential impacts, and to present options for mitigating them, as well as for maximising opportunities to use sport as a way to promote and enhance biodiversity conservation.