This report addresses study area delineation and site selection for the Pangani Environmental Flow Assessment work. The task was accomplished by first separately delineating relatively homogeneous zones and sub-zones along the river and estuary, and for socio-economic activities in the basin. Thereafter, the resultant delineations were synchronised to produce a scheme for all project work. Within this scheme, representative sites were selected in the subzones deemed to be most relevant to the study.